Catalase Lab Report

Investigating the effect of antithetical environmental factors on the reaction of colorful and atomic number 1 hydrogen peroxide turn off of Contents 1 Design3 1. 1 Variables3 1. 2 pencil eraser and Environment3 2 info Collection and Analysis3 2. 1 Collected Raw entropy3 2. 2 Qualitative data5 2. 3 Processed data5 2. 4 chart on test tube 25 2. 5 Graph on test tube 36 2. 6 Errors6 3. Conclusion and Evaluation6 3. 1. Conclusion6 3. 2. Evaluation7 3. 2. 1. hit-or-miss Errors7 3. 2. 2. Systematic Errors7 3. 3. Improvements7 Design Look to sheet call Investigating the action of the enzyme catalase 1 1. 1 Variables The independent variables are the acids used, the qualified variables the height of the bubbles formed and the control variables the test tubes used. 2 1. 2 Safety and Environment The safety and environmental precautions for this lab are quite strict. fracture safety goggles and a lab coat to avoid give-up the ghost any acid on your person, and dispose of the acid s in a pass away with plenty of water.Collect the used liver proves and dispose of accordingly. 2 Data Collection and Analysis 1 2. 1 Collected Raw Data Test tube Height of bubbles (cm) pH 30s 30s 60s 90s 120s 150s 180s 210s 240s 270s 300s 1 0 0 0 0 0 0 0 0 0 0 2 12,9 13 14,1 13,3 11,7 10,4 9,3 6,7 5,5 5 3 14,2 10,8 8,4 6,9 5,5 7,5 3,3 3 2,8 2,7 4 1,9 1,9 1,8 1,4 1,3 1,3 0,4 0,2 0,1 0,1 5 0,2 0,2 0,2 0,1 0,1 0,1 0,1 0,1 0,1 0,1 6 0,5 0,6 0,9 1 1,4 1,6 1,8 2 1,3 1,4 2 2. 4 Graph on test tube 2 3 4 2. 5 Graph on test tube 3As fucking be seen from the two above graphs, test tube 3, which had the exact very(prenominal) conditions as test tueb 2 except for the surface area of the liver, had a much more than vigourous reaction, due to the increased surface area of the impoverished liver. 2. 6 Errors The only piece of equipment that is to be considered in error calculations is the swayer used which had an uncertainty of approx. 0,2 cm. 3. Conclusion and Evaluation 3 . 1. Conclusion The experiment was meant to give an artificial catalase reaction between the enzymes in the liver sample and hydrogen peroxide.The sample in test tube 2 reacted with the hydrogen peroxide and produced oxygen bubbles at a somewhat steady pace, while the sample in test tube 3 reacted vigorously at start-off and then slowed stilt as most of the enzyme had been used up. 3. 2. Evaluation 3. 2. 1. Random Errors The liver was quite hard to get into perfect ? cm3 cubes, as it tended to get squished when the knife was pressed down, making the sample larger lengthwise, but small height-wise.Also when the reaction was very rigorous, the oxygen bubbles sometimes lifted the liver out of the hydrogen peroxide, causing it to stop reacting with the hydrogen peroxide. While non a major issue, sometimes the time at which the results were canvass were non exactly at the designated 30 second intervals, due to many things leaving on at once. 3. 2. 2. Systematic Errors The ruler w e were using was quite quondam(a) and dirty, with some of the finer millimeter markings rubbed off or obscured, leading to readings that were not as accurate as they could have been.The molarity of the chemicals used puke also be put into question. 3. 3. Improvements The liver could be frozen or in some other way petrified to make the cutting easier and more precise. The liver should be checked constantly and adjusted back down with a glass rod if necessary. Enough time should be allocated to insure that the experiment can be done in a solace and orderly fashion to avoid any oversights in the time taking. absolved and clear rulers should be used to measure the bubbles. The molarity of the chemicals should be checked with titration or some other form of double checking the molarity.